Interaction between Cytochrome c iso-1 (5cie_D) and Cytochrome c peroxidase, mitochondrial (5cie_A)
PDB and SCOP data
PDB ID: 5cie (all binary interactions in this PDB entry)
Title: Complex of yeast cytochrome c peroxidase (W191G) bound to aniline with iso-1 cytochrome c
Release date: 2016-08-03
Resolution: 2.6 Å
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Chain A: 5cie_D
Title: Cytochrome c iso-1
Source organism: Saccharomyces cerevisiae S288C
Number of residues: 108
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Chain B: 5cie_A
Title: Cytochrome c peroxidase, mitochondrial
Source organism: Saccharomyces cerevisiae S288C
Number of residues: 294
Structural properties
Hetero-dimer interface properties
Buried interface area: 143.25 Å2
Number of inter-residue contacts at the interface: 12
Number of H-bonds: 1
Number of salt bridges: 2
Experimental structure
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Pairwise interaction
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Biological assembly
Heteromer, 4 proteins.
Interface structure modeling
Residues at the interaction interface
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Interface residues in 5cie_D
No. Residue no. in structure Residue no. in sequence Residue name Buried ASA, Å2 Buried ASA, % 1 -5 1 T 3.0 1.9 % 2 -4 2 E 37.3 24.2 % 3 62 67 N 3.2 4.3 % 4 66 71 E 15.0 17.8 % 5 86 91 K 14.8 11.6 % 6 87 92 K 13.3 11.0 % 7 88 93 E 56.5 48.1 %
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Interface residues in 5cie_A
No. Residue no. in structure Residue no. in sequence Residue name Buried ASA, Å2 Buried ASA, % 1 29 29 K 17.0 45.2 % 2 32 32 E 43.0 37.3 % 3 33 33 D 15.6 34.5 % 4 90 90 K 30.3 22.0 % 5 93 93 E 2.9 7.8 % 6 94 94 P 9.0 13.9 % 7 97 97 K 25.5 15.5 %
Inter-residue contacts at the interaction interface
No. | Residue no. in chain A structure | Residue no. in chain A sequence | Residue in chain A | Residue no. in chain B structure | Residue no. in chain B sequence | Residue in chain B | Contact area, Å2 | Contact type |
---|---|---|---|---|---|---|---|---|
1 | -5 | 1 | T | 90 | 90 | K | 3.0 | |
2 | -4 | 2 | E | 90 | 90 | K | 27.2 | H-bond, Salt bridge |
3 | -4 | 2 | E | 93 | 93 | E | 2.9 | |
4 | -4 | 2 | E | 97 | 97 | K | 7.2 | |
5 | 62 | 67 | N | 97 | 97 | K | 3.2 | |
6 | 66 | 71 | E | 97 | 97 | K | 15.0 | |
7 | 86 | 91 | K | 32 | 32 | E | 14.8 | |
8 | 87 | 92 | K | 32 | 32 | E | 13.3 | |
9 | 88 | 93 | E | 29 | 29 | K | 17.0 | Salt bridge |
10 | 88 | 93 | E | 32 | 32 | E | 14.9 | |
11 | 88 | 93 | E | 33 | 33 | D | 15.6 | |
12 | 88 | 93 | E | 94 | 94 | P | 9.0 |
Sequence alignments
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Query protein: sp|P00044|CYC1_YEAST Cytochrome c iso-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CYC1 PE=1 SV=2
Result domain: 5cie_D; Cytochrome c iso-1
Alignment data:
Expectation value = 1.54e-77, Score = 226 bits (575),
Identities = 100% (108/108), Positive = 100% (108/108), Gaps = 0% (0/108).
Interface alignment data:
Interface residues in alignment: 100% (7/7).
Identities = 100% (7/7), Positive = 100% (7/7), Gaps = 0% (0/7).
Query: 2 TEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGRHSGQAEGYSYTDANIKK 61
TEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGRHSGQAEGYSYTDANIKK
5cie_D: 1 TEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGRHSGQAEGYSYTDANIKK 60
dssp: -------HHHHHHHHHHH------------------------------------HHHHHH
Query: 62 NVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLITYLKKACE 109
NVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLITYLKKACE
5cie_D: 61 NVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLITYLKKACE 108
dssp: --------HHHH--------------------HHHHHHHHHHHHH---
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Query protein: sp|P00431|CCPR_YEAST Cytochrome c peroxidase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CCP1 PE=1 SV=2
Result domain: 5cie_A; Cytochrome c peroxidase, mitochondrial
Alignment data:
Expectation value = 0, Score = 607 bits (1564),
Identities = 99% (291/294), Positive = 99% (291/294), Gaps = 0% (0/294).
Interface alignment data:
Interface residues in alignment: 100% (7/7).
Identities = 100% (7/7), Positive = 100% (7/7), Gaps = 0% (0/7).
Query: 68 TTPLVHVASVEKGRSYEDFQKVYNAIALKLREDDEYDNYIGYGPVLVRLAWHTSGTWDKH 127
TTPLVHVASVEKGRSYEDFQKVYNAIALKLREDDEYDNYIGYGPVLVRLAWH SGTWDKH
5cie_A: 1 TTPLVHVASVEKGRSYEDFQKVYNAIALKLREDDEYDNYIGYGPVLVRLAWHISGTWDKH 60
dssp: ------E--------HHHHHHHHHHHHHHHHH---HHHH---HHHHHHHHHHHH---E--
Query: 128 DNTGGSYGGTYRFKKEFNDPSNAGLQNGFKFLEPIHKEFPWISSGDLFSLGGVTAVQEMQ 187
DNTGGSYGGTYRFKKEFNDPSNAGLQNGFKFLEPIHKEFPWISSGDLFSLGGVTAVQEMQ
5cie_A: 61 DNTGGSYGGTYRFKKEFNDPSNAGLQNGFKFLEPIHKEFPWISSGDLFSLGGVTAVQEMQ 120
dssp: --E----------HHHH--------HHHHHHHHHHHHH-----HHHHHHHHHHHHHHH--
Query: 188 GPKIPWRCGRVDTPEDTTPDNGRLPDADKDADYVRTFFQRLNMNDREVVALMGAHALGKT 247
GPKIPWRCGRVDTPEDTTPDNGRLPDADKDA YVRTFFQRLNMNDREVVALMGAHALGKT
5cie_A: 121 GPKIPWRCGRVDTPEDTTPDNGRLPDADKDAGYVRTFFQRLNMNDREVVALMGAHALGKT 180
dssp: -----E--------HHH-------------HHHHHHHHH-----HHHHHHHHHHHH--EE
Query: 248 HLKNSGYEGPWGAANNVFTNEFYLNLLNEDWKLEKNDANNEQWDSKSGYMMLPTDYSLIQ 307
HLKNSGYEGP GAANNVFTNEFYLNLLNEDWKLEKNDANNEQWDSKSGYMMLPTDYSLIQ
5cie_A: 181 HLKNSGYEGPGGAANNVFTNEFYLNLLNEDWKLEKNDANNEQWDSKSGYMMLPTDYSLIQ 240
dssp: --------EE----------HHHHHHHH--EEEEE-----EEEEE----EE-HHHH----
Query: 308 DPKYLSIVKEYANDQDKFFKDFSKAFEKLLENGITFPKDAPSPFIFKTLEEQGL 361
DPKYLSIVKEYANDQDKFFKDFSKAFEKLLENGITFPKDAPSPFIFKTLEEQGL
5cie_A: 241 DPKYLSIVKEYANDQDKFFKDFSKAFEKLLENGITFPKDAPSPFIFKTLEEQGL 294
dssp: -HHHHHHHHHHHH-HHHHHHHHHHHHHHHHH---E--------E----------